TCTP interactomics in NF1-associated malignant tumor cells by affinity-purification and SWATH-MS

Abstract

Neurofibromatosis type 1 (NF1) is an autosomal dominant RASopathy disease that predisposes individuals to developing benign neurofibromas and malignant peripheral nerve sheath tumors (MPNST). The mechanisms of NF1-tumorigenesis or the radical curatives have not been established. Using a unique transcriptome and proteome integration method, iPEACH^1–4, we previously identified translationally controlled tumor protein (TCTP) as a novel biological target for NF1-associated tumors⁵. To evaluate the function of TCTP in NF1-associated malignant tumor cells, the cellular interactome of TCTP, overexpressed as Flag-tagged protein, was identified by affinity-purification using anti-FLAG antibody coupled with SWATH-MS analysis (AP-SWATH). The data described in this paper have been deposited to jPOST^6,7 with the identifier JPST000107.