Dataset of SARS-CoV-2-infected cells and how it can optimize whole viral particle antigen production for vaccines

Abstract

This study aimed to elucidate the dynamics of whole-virion antigen production for vaccine development and was conducted at the very early stage of the COVID-19 pandemics. Vero E6 cells infected with the SARS-CoV-2 Italy-INMI1 isolate were collected at 1, 2, 3, 4, and 7 days post-infection under two multiplicities of infection (MOIs). Two biological replicates were systematically analyzed. The samples were subjected to in-gel tryptic digestion and analyzed by data-dependent acquisition (DDA) mass spectrometry. The aim of this study is to summarize sample preparation, DDA data acquisition, and their analysis.