Dataset for the PHD3-mediated Hydroxylation Site Analysis in HIV-2 Vpx

Abstract

The dataset (JPST003527) reports LC-MS/MS analyses performed to investigate the hydroxylation of Pro41 in human immunodeficiency virus type 2 (HIV-2) viral protein X (Vpx) by prolyl hydroxylase domain-containing protein 3 (PHD3). In vitro hydroxylation assays using synthetic Vpx peptide (residues 31-48) and recombinant PHD3 protein demonstrated selective hydroxylation of peptides containing Pro41. In addition, Chemical cross‑linking MS dataset (JPST003526) of the Vpx-PHD3 complex further provided structural information on their interaction. Collectively, these data support site‑specific proline hydroxylation in Vpx and offer insights into its association with PHD3.