Quantitative relationship between insecticidal activity and Ca²⁺ pump stimulation by flubendiamide and its related compounds

Abstract

Flubendiamide is the first benzenedicarboxamide compound developed as an insecticide with potent activity against lepidopterous pests. It has already been clarified that insecticidal activity of the compound is mediated through modulation of the ryanodine-sensitive Ca²⁺ release channel, (ryanodine receptors; RyRs). The present study showed close correlation between insecticidal activities and stimulating effects on the Ca²⁺ pump, a functional co-operative element with RyR activity, and implied the involvement of Ca²⁺ pump stimulation in insecticidal activity. This result suggested that Ca²⁺ pump activity could be a convenient indicator for determining RyR activity.