Purification and cDNA cloning of a defensin in Brassica juncea, its functional expression in Escherichia coli, and assessment of its antifungal activity

Yoshiyuki Sagehashi; Yoshifumi Oguro; Takashi Tochihara; Tetsuo Oikawa; Hiroshi Tanaka; Motoshige Kawata; Masamichi Takagi; Osamu Yatou; Hiroaki Takaku

doi:10.1584/jpestics.D12-033

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Purification and cDNA cloning of a defensin in Brassica juncea, its functional expression in Escherichia coli, and assessment of its antifungal activity

Yoshiyuki Sagehashi, Yoshifumi Oguro, Takashi Tochihara, Tetsuo Oikawa, Hiroshi Tanaka, Motoshige Kawata, Masamichi Takagi, Osamu Yatou, Hiroaki Takaku

著者情報

キーワード: defensin, antifungal peptide, Brassica juncea, Magnaporthe oryzae

ジャーナルフリー早期公開

論文ID: D12-033

DOI https://doi.org/10.1584/jpestics.D12-033

この記事には本公開記事があります。

The final version of this article is now available: Vol. 38 (2012), No. 1

詳細

抄録

We describe the purification, cDNA cloning, and characterization of a defensin, AFP1, in Brassica juncea, which shares 100% amino acid sequence identity with Raphanus sativus defensin (Rs-AFP1) and has a high antifungal activity against Magnaporthe oryzae. The recombinant AFP1 synthesized in Escherichia coli showed thermostability and antifungal activity against a broad spectrum of rice pathogenic fungi. The changes of a negative to positive charge at the surface of AFP1 derived by amino acid substitutions showed more enhanced antifungal activities than the wild-type AFP1.

責任著者(Corresponding author)

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