Effects of Edaravone on Singlet Oxygen Released From Activated Human Neutrophils

Abstract

The effects of edaravone, a curative agent for acute brain infarction, on singlet oxygen (¹O₂) released from activated human neutrophils were examined, and the effects were compared to those of histidine, a ¹O₂ singlet oxygen scavenger. The neutrophils, stimulated with opsonized zymosan, released ¹O₂ that was detected by chemiluminescence using a ¹O₂ specific probe, trans-1-(2'-methoxyvinyl)pyrene. Edaravone dose-dependently suppressed the ¹O₂ release with an IC₅₀ of approximately 0.3 μM, while the IC₅₀ of histidine was approximately 1 mM. This ¹O₂ scavenging activity of edaravone might be involved in its curative effects on acute brain infarction.