Journal of Pharmacological Sciences
Online ISSN : 1347-8648
Print ISSN : 1347-8613
ISSN-L : 1347-8613
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Possible Underlying Mechanism for Hydrogen Peroxide-Induced Electromechanical Suppression in Human Atrial Myocardium
Shih-Hurng LohJong-Shiaw JinChien-Sung TsaiChao-Ming ChaoYin TsaiWei-Hwa ChenTzu-Hurng ChengChao-Chin ChuangCheng-I Lin
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2003 Volume 91 Issue 1 Pages 53-60

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Abstract

Hydrogen peroxide (H2O2) and its metabolites have been shown to exert complex effects on the cardiac muscle during cardiac ischemia/reperfusion. The aim of the present study, by perfusing H2O2 or/and different scavengers of oxygen free radicals (OFRs) into the human atrium, is to characterize the electropharmacological effects of H2O2 and explore its possible underlying mechanism. Atrial tissues obtained from the heart of 19 patients undergoing corrective cardiac surgery were used. Transmembrane action potentials were recorded using the conventional microelectrode technique, and contraction of atrial fibers was evaluated in normal [K]o (4 mM) in the absence and presence of tested agents. H2O2 (30 μM-3 mM) had a biphasic effect on the contractile force (an increase, followed by a decrease), reduced the 0-phase depolarizing slope (dV/dt), and prolonged the action potential duration (APD) in a concentration-dependent manner. However, even at a concentration as high as 3 mM, H2O2 did not influence diastolic membrane potential (DMP). Pretreatment with N-(mercaptopropionyl)-glycine (N-MPG), a specific scavenger of the · OH free radical, significantly blocked the 3 mM H2O2-induced electromechanical changes, while the pretreatment with L-methionine (L-M), a specific scavenger of HOCl free radical, did not. Our data suggests that the toxic effects of H2O2 are caused mainly through the generation of · OH, which is attributed to the electropharmacological inhibitory effects seen in the human atrium.

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© The Japanese Pharmacological Society 2003
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