Effects of 4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic Acid (DIDS) and Chlorpromazine on NO3− Transport via Anion Exchanger in Erythrocytes: Inertness of DIDS in Whole Blood

Kaname Kubota; Takaharu Ishibashi; Taku Matsubara; Tomoyuki Hori; Kazuyuki Ozaki; Masaru Yamazoe; Junko Yoshida; Matomo Nishio; Yoshifusa Aizawa

doi:10.1254/jphs.93.505

Short Communications

Effects of 4,4'-Diisothiocyanato-stilbene-2,2'-disulfonic Acid (DIDS) and Chlorpromazine on NO₃⁻ Transport via Anion Exchanger in Erythrocytes: Inertness of DIDS in Whole Blood

Kaname Kubota, Takaharu Ishibashi, Taku Matsubara, Tomoyuki Hori, Kazuyuki Ozaki, Masaru Yamazoe, Junko Yoshida, Matomo Nishio, Yoshifusa Aizawa

Author information

Kaname Kubota
Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences
Takaharu Ishibashi
Department of Pharmacology, Kanazawa Medical University
Taku Matsubara
Division of Cardiology, Shinrakuen Hospital
Tomoyuki Hori
Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences
Kazuyuki Ozaki
Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences
Masaru Yamazoe
Division of General Medicine, Niigata City General Hospital
Junko Yoshida
Department of Pharmacology, Kanazawa Medical University
Matomo Nishio
Department of Pharmacology, Kanazawa Medical University
Yoshifusa Aizawa
Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences

Keywords: NO₃⁻, erythrocyte (RBC), DIDS

JOURNAL FREE ACCESS

2003 Volume 93 Issue 4 Pages 505-508

DOI https://doi.org/10.1254/jphs.93.505

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Abstract

We examined the effects of chlorpromazine on NO₃⁻ transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 μg/ml) did not influence NO₃⁻ movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor DIDS (4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid, 100 μM) did not alter NO₃⁻ movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO₃⁻ transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma.

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