Abstract
The effects of NaNO2 and NH2OH on mitochondrial MAO from rat brain were studied with the following results: NaNO2 and NH2OH activated the enzyme. The effects of NaNO2 and NH2OH on enzyme activity differed with different substrates. The activating effects of NaNO2 and NH2OH on MAO activity were reversible. NaNO2 did not cause activation at any concentration of pheniprazine and pargyline tested. However, 1×10-2M NH2OH decreased the inhibitions by pheniprazine and pargyline at all concentrations of the inhibitors tested. Addition of 1×10-2M NH2OH changed the Km value of MAO for tyramine from 1.25×10-2M to 6.2×10-3M.
