Functional Expression of H₁-Histaminergic Receptors in Xenopus Laevis Oocytes Injected with Bovine Adrenal Medullary mRNA

Abstract

We prepared poly (A)⁺ mRNA from bovine adrenal medulla and electro physiologically examined the expression of H₁-histaminergic receptors in Xenopus laevis oocytes. Histamine dose-dependently induced inward currents in oocytes injected with the mRNA, but not in those injected with water or the non-injected oocytes. This response was also induced by H₁-agonists and antagonized by H₁-antagonists, while both an H₂-agonist and an antagonist had no effect. These results clearly demonstrate that H₁-histaminergic receptors are functionally expressed in oocytes injected with exogenous mRNA.