The Japanese Journal of Pharmacology
Online ISSN : 1347-3506
Print ISSN : 0021-5198
ISSN-L : 0021-5198
Prostanoid Receptor-Mediated Calcium Signaling in Cultured Rat Astrocytes
Junichi KitanakaKazuhiro TakumaKaoru KondoAkemichi Baba
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1996 Volume 71 Issue 1 Pages 85-87

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Abstract

We investigated prostanoid-induced intracellular Ca2+ mobilization in Ca2+-sensitive dye fura-2-loaded cultured astrocytes. The thromboxane (TX) A2 analog STA2 (9, 11-epithio-11, 12-methanoTXA2) and/or prostaglandin (PG) F (each used at 1 μM) stimulated intracellular Ca2+ mobilization in single cultured rat type 1 astrocytes. Three response patterns were observed: only STA2-sensitive, only PGF-sensitive, and both prostanoids-sensitive cells. The Ca2+ response was prostanoid-dose-dependent (0.1-1 μM) and showed a rapid spike-like Ca2+ rise that peaked within 30 sec after the stimulation by the ligand. These observations suggest that type 1 astrocytes are heterogeneous with respect to the expression of receptors for TXA2 and PGF, which are linked to Ca2+ mobilization.

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