1939 年 53 巻 633 号 p. 415-420
About a month old mixture of absolute alcohol and carbon tetrachloride reacts strongly acid (pH ca. 1), owing to the formation of hydrochloric acid. If we employ such a acid mixture as intermedium for the paraffin imbedding of fixed materials (root tips and anthers of Vicia Faba, Lilium longiflorum, Reineckia carnea, Fritillaria verticillata and Tradescantia virginica), the nucleal reaction of the karyotin and chromosomes in the material is found to be negative.
The same is the case, if we treat the material with 1% alcoholic solution of HCl. On the other hand we found the positive necleal reaction of the karyotin in the material treated with the mixture of absolute alcohol and other acids (nitric, suuphuric and acetic acid) or ethylene chloride.