植物学雑誌
Online ISSN : 2185-3835
Print ISSN : 0006-808X
ISSN-L : 0006-808X
生體染色に於ける液胞内色素分離の機作
澤井 輝男
著者情報
ジャーナル フリー

1950 年 63 巻 750 号 p. 270-277

詳細
抄録

1. In the plant cell vacuoles, preliminarily stained in vivo with thiazine dyes (but excepting methylene blue), there appear numerous crystals of the dyestuffs, when they are treated with the solutions of thiocyanide, iodide, bromide, chloride or nitrate of alkali metals (Na, K, or Ca). These demixed crystals show various types of spiral shape.
2. This demixing reaction is accelerated with the increase of the concentration of the dye-solution used, but also related to the sort of the anion of the salt employed, giving the following anion-series, concerning the acceleration of the reaction:
SCN->I->Br->NO3->Cl-
3. The same demixing reaction is proved to take place in the mere water solutions of the dyestuffs, when they are treated with solutions of the same salts through a semipermeable membrane of collodion or pig bladder. The anion-series obtained from these model experiments perfectly coincide with the one obtained from the vital stainings of cell vacuoles.
4. It may be concluded, therefore, that the demixing of the spiral formed thiazine dyes in plant vacuoles has been aroused by a simple reaction of salting-out of dyecrystals in solutions. Although their shapes were various and often curious, no parts would be played by the constituents of cell saps or cytoplasms.

著者関連情報
© 公益社団法人 日本植物学会
前の記事 次の記事
feedback
Top