ハシリドコロのポリフェノラーゼの研究I.
カテコールの酸化について
1955 年 68 巻 807 号 p. 227-232
詳細
1955 年 68 巻 807 号 p. 227-232
1. A crude polyphenolase has been extracted from the subterranean stem of Scopolia japonica, into the phosphate buffer.
2. In the presence of this enzyme catechol takes up oxygen at a high rate, DL-dihydroxyphenyl-alanine and pyrogallol are oxidized rapidly, phloroglucinol and hydroquinone rather slowly, while resorcinol, L-tyrosine, phenol and p-cresol are not oxidized at all.
3. Among the inhibitors of the oxidation of catechol, cyanide is very effective, but sodium diethyldithiocarbamate is less effective. The author wishes to thank Prof. M. Shibata of the Biological Institute and Prof. K. Fukui of the Chemical Institute, Toyama University, for their great interests in this work. The material used in the present study was supplied by Prof. T. Nakaoki of the Faculty of Pharmacy, Toyama University, to whom his cordial thanks are due.
