植物組織におけるTTC還元について補足的報告

抄録

The effects of arsenite, pyrophosphate, citrate, pH, 2. 4-D, thickness of slices and cyanide on the TTC reduction in the slices of radicle and hypocotyl of seedlings of Phaseolus vulgaris were examined. Among these reagents and reaction conditions, arsenite, pyrophosphate, cyanide and low pH inhibited the reduction of TTC, while 2. 4-D accelerated it slightly. Citrate had no appearent effect on it. When the slices were thinner than about 150μ no reduction appeared in them even under the best conditions. The inhibition by cyanide and its partial reqersibility were confirmed. It was concluded that the TTC method for the histochemical demonstration of succinic dehydrogenase in plant tissues is reliable, and that the enzyme is distributed in the cells of procambial tissue.