染色法による羊歯類前葉体特に精子の荷電と rH の測定

抄録

To make clear the electric charge and the rH, the staining reactions by the methods of Unna-Golodetz's neutral violet extra, prussian blue, Mn-methyl green, and rH-indicators were tested to the spermatozoid (spermatid) or the prothallium in fern. The following facts were gotten from the results of staining reactions.
(1) The nucleus of spermatozoid shows the negative charge but the cilia-bearing band, the cilium, and the border-brim do not show it. The cytoplasm of the spermatid also show the negative charge.
(2) In the prothallium, the cells of the meristemal portion and the nucleus of prothallial cell, and the rhizoidal cell present the negative charges, whereas the cytoplasm of prothallial cells show the positive charge, or the posive and the negative charges locally. As to the charge, the polarity is thought to exist in the whole area of prothallium.
(3) The nucleus of spermatozoid and the cytoplasm of spermatid show the oxidation color and their rHs are high. In the prothallium, the meristemal portion presents often the oxidation color, whereas the prothallial cell (cytoplasm, membrane) and the rhizoid (cytoplasm) show the reduction color and their rHs are low. Judging from these facts the polar property of the prothallium is thought to exist.