花粉の生理学的研究XIII

Camellia japonica の花粉管の伸長阻害について

抄録

1) Elongating pollen tubes ofCamellia japonica were checked by putting a slice of mature stigma ofLilium longiflorum on their culture plate. From this it is inferred that the stigma contains some substances which inhibit the growth of Camelliapollen tube and the quantity of these substances increases during the maturation of Lilium stigma (Fig. 3).
2) WhenCamelliapollen tube is affected in their growth, the tube elongation and protoplasmic streaming become intermittent, and the “Cap brock “ frequently appears out of shifted from the centre of the tip of pollen tube (Fig. 5, 6).
3) The, separation of the substances in the stigma ofLiliumwhich can inhibit the growth ofCamelliapollen was demonstrated by paper chromatography and pollen germination test. The Rf-value of one of these substances was found to be 0.2-0.25, usingn-butanol, ethanol and water in a ratio of 4: 1: 1 is developer (Fig. 7).