Young pea stem sections were treated by 2, 4-dichloroanisole (DCA), malefic hydrazide (MH), coumarin, p-chloromercuribenzoate (PCMB), monoiodoacetic acid and 2, 4-dinitrophenol (DNP), in combination with IAA or without it.
Promotion and inhibition of water absorption due to various concentrations of those substances were amazingly paralleled by pectin methylesterase (PME) activity of the homogenate of the treated tissue. Compared with other substances, DNP inhibited water absorption relatively more severely than PME activity.
The effects of substances on the PME activity in the case of tissue treatment were not due to their direct effects on the enzyme preparation.
A hypothesis is proposed that activated PME increases extensibility of walls of growing cells, and consequently their water absorption.