1959 Volume 72 Issue 850 Pages 146-151
The morphogenesis of the isolated internodal cell of Nitella flexilis cultured in vitro was promoted by application of adenylic acid, gibberellin and most amino acids, e.g., leucine, methionine, glutamic acid, aspartic acid and arginine in appropriate concentrations. Amino acids had a positive effect on the formation and the elongation of both the shoot and rhizoid. Adenylic acid was favorable for shoot and rhizoid formation. Gibberellin was suitable for the elongation of the shoot.
Colchicine exerted no effect on formation of shoot and rhizoid at whatever concentrations, but it could accelerate the elongation of both the shoot and rhizoid already formed.