赤血球懸濁液の超音波測定による膜の相転移

抄録

We have measured the ultrasonic velocity V and the density ρ of swine intact erythrocytes and ghosts suspended in a physiological saline solution over the temperature range from 20° to 40°C, by using an automatic measuring system which mainly consists of a vibrating densimeter and a sequential pulse oscillation velocimeter improved from the sing-around method. The behavior of the temperature dependence of V, ρ and the compressibility B=1/ρ V² in the erythrocyte suspension has a close similarity to those in the ghost suspension; near 30°C, the limiting density number [ρ], the limiting velocity number [V] and the limiting compressibility number [B] decreased largely both in the erythrocyte suspension and in the ghost suspension, where [A]=_??_, A is V, ρ or B, and H is the volume fraction of erythrocytes or the dry weight concentration of ghosts. The density and the compressibility of the whole erythrocyte and the ghost membrane are obtained; their temperature dependence is discussed. ( J. Jpn. Soc. Biorheol., 1 (1), 38~45, 1987.)