Abstract
Glomerular thrombosis was successfully induced in rats by the injection of 0.2 ml of nephrotoxic antiserum 24 hours prior to the injection of 0.02 mg of lipopolysaccharide. All of experimental rats showed fibrin deposit in glomerular capillaries by the evaluation of both hematoxylin-eosin and fluorescent stainings, however, any thromboembolic lesions were not present in other organs such as lung, liver and intestine. Since some of these rats could be alive more than 60 days, it was possible to evaluate the long-term histo-logical changes after the induction of experimental glomerular thrombosis. Fibrin deposit was transient, and localized in segmental within one week. The number of circulating leukocytes and platelets, the value of plasma fibrinogen and Factor VIII activity were rapidly decreased during first 7 hours and recovered within 24 hours, while the value of serum FDP showed the transient increase. After 5 days of acute fibrin deposit, cell proliferation in glomeruli and crescent for-mation appeared and increased progressively until 2 months later, when 50% of glomeruli showed crescent formation. The experimental glomerular thrombosis can be a useful model for the evaluation of the pathogenesis of glomerular proliferation including crescent formation as well as glomerular thrombosis.
