The Japanese Journal of Urology
Online ISSN : 1884-7110
Print ISSN : 0021-5287
ISSN-L : 0021-5287
IN VITRO ANTIPROLIFERATIVE EFFECT OF CANCER CHEMOTHERAPEUTIC AGENTS AND THEIR COMBINATION WITH INTERFERON ON RENAL CELL CARCINOMA
Taiji TsukamotoYoshiaki KumamotoNoriomi MiyaoKiyotaka OmuraKiyohito Yamazaki
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1989 Volume 80 Issue 1 Pages 88-94

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Abstract

We studied an antiproliferative effect of cancer chemotherapeutic agents, interferon (IFN) and, in particular, their combination effect on renal cell carcinoma.
Either of colony formation assay (CFA) or [3H]-thymidine incorporation assay ([3H]-TdR assay) was employed as an in vitro chemosensitivity testing system. When compared, these two systems produced similar results with a good correlation (r=0.97, p<0.01), in the antiproliferative effect of 30 drugs for 4 primary renal cell carcinomas and 5 xenotransplantable renal cell carcinomas.
In vitro chemosensitivity test (CFA or [3H]-TdR assay) screened successfully only 5 “sensitive” drugs (7.9%) out of a total 63 cancer chemotherapeutic agents for 24 human renal cell carcinoma. This comfirmed the findings reported by others.
In the study of the antiproliferative effect of a cancer chemotherapeutic agent, human lymphoblastoid interferon (HLBI) and their combination on human renal cel carcinoma cell line (SMKR2). Each of VBL, MTX or HLBI tended to suppress [3H]-TdR uptake in a dose-dpendent manner. The combination of VBL (0.05μg/ml) and HLBI (102 or 103IU/ml) produced a subadditive effect, and that of MTX (0.1μg/ml) and HLBI 102IU/ml produced a synergistic effect on the human renal cell carcinoma cell line, the effect which is evaluated by Valeriote and Lin's criteria of combination. In particular, the synergistic effect by MTX and HLBI under the clinically achievable drug concentration seems important, when its clinical application is considered.

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