The Japanese Journal of Urology
Online ISSN : 1884-7110
Print ISSN : 0021-5287
ISSN-L : 0021-5287
ISOLATION OF URINARY HEPARAN SULFATE AND CHONDROITIN SULFATE AND THEIR INHIBITORY ACTIVITY OF CALCIUM OXALATE CRYSTAL GROWTH
Hidenobu IwataTakashi TeradoMasahiro KinShunji NishioMasafumi TakeuchiAtsushi Matsumoto
Author information
JOURNALS FREE ACCESS

1991 Volume 82 Issue 3 Pages 405-411

Details
Abstract

Urinary heparan sulfate (U-HS) and chondroitin sulfate (U-ChS) were isolated and partially purified using two steps of anion-exchange chromatography (DE-52 and Dowex 1×2). Uronic acid content of these urinary glycosaminoglycans (GAGs) and commercial heparan sulfate (C-HS) and chondroitin-6-sulfate (C-C6S) was as follows; U-HS: 22.4%, U-ChS: 32.0%, C-HS: 41.6%, C-C6S: 36.0%.
The inhibitory activity of these GAGs on calcium oxalate crystal growth was examined using the crystal-seed system described by Koide et al. The influence of uric acid on the inhibitory activity of the GAGs was also studied. U-HS and U-ChS exhibited nearly equal inhibitory activity to their respective commercial preparations. The inhibitory activity of U-HS was stronger than that of U-ChS. Uric acid suppressed the inhibitory activity of both, and the degree of suppression was stronger in U-ChS than in U-HS.
The molecular weight of the urinary GAGs was compared to that of the commercial chondroitin-6-sulfate (C6S; m. w. between 40, 000 and 80, 000) by gel-filtration using Sephacryl S-300HR. C6S was eluted slightly behind the void volume. U-ChS was eluted much behind C6S. Therefore, the molecular weight of U-ChS was thought to be much smaller than that of C6S. U-HS was eluted into two peaks; the first one at the void volume and the second one between the peaks of C6S and U-ChS. These findings indicate the possibility that part of U-HS exists in the form of proteoglycan.

Information related to the author
© Japanese Urological Association
Previous article Next article
feedback
Top