1991 Volume 82 Issue 7 Pages 1150-1156
The hCGβ-like immunoreactivity (IR-hCGβ) in the sera and urine of three patients with transitional cell carcinoma of the bladder was charcterized by enzyme-immunoassay which specifically recognized the hCGβ-related molecular species (whole hCG, free hCGβ and β-core fragment). There was a qualitative difference in IR-hCGβ between the serum and the urine in all of the patients. Free hCGβ was exclusively detected in the serum, whereas both free hCGβ and β-core fragment (β-CF) could be detected in the urine. Whole hCG could not be virtually observed in the sera and the urine from all the patients examined. The concentrations of urinary β-CF were consistently much higher than those of urinary and serum free hCGβ in all the patients. The immunohistochemical study was performed by using two kinds of the antibody against the core and the carboxyterminal portions of the hCGβ molecule. Immunoperoxidase staining was positive not only in the syncytiotrophoblastic giant cells but also in the trasnsitional cell carcinoma cells for both antibodies.
The present results suggest that free hCGβ is secreted from the tumor cells and metabolized into β-CF may provide a new index to detect a transitional cell carcinoma of the bladder.