The Japanese Journal of Urology
Online ISSN : 1884-7110
Print ISSN : 0021-5287
ISSN-L : 0021-5287
GENE EXPRESSIONS OF TYPE IV COLLAGENASE AND TISSUE INHIBITOR OF METALLOPROTEINASES (TIMP) IN HUMAN BLADDER CANCERS
Seiichi NaruoHiroomi KanayamaMasashi AkiSusumu Kagawa
Author information
JOURNALS FREE ACCESS

1993 Volume 84 Issue 5 Pages 841-850

Details
Abstract

Matrixmetalloproteinases (MMP), such as type IV collagenases and interstitial collagenases, play an important role in tumor invasion and metastasis. And tissue inhibitor of metalloproteinases (TIMP) inhibit collagenolytic activity of these enzymes. We investigated the gene expressions of MMP-9 (92kDa type IV collagenase), MMP-2 (72kDa type IV collagenase), TIMP-1 and TIMP-2 in bladder cancers by Northern blot and slot blot hybridization. The mRNA levels of MMP-2, TIMP-1 and TIMP-2 increased in the cases with invasion and metastasis of bladder cancers. These findings suggest that MMP-2 acts as a regulator of the invasion and metastasis of bladder cancers. The MMP-2/TIMP-2 ratio increased as tumor invasion and metastasis progressed, suggesting that an imbalance in the MMP and TIMP ratio promote the invasion and metastasis of bladder cancers. And we also investigated the gene expressions of c-fos that activate the collagenase genes, and there was a correlation between c-fos and MMP-2 in gene expressions. It is suggested that fos gene may play an important role for the invasion and metastasis in bladder cancers.

Information related to the author
© Japanese Urological Association
Previous article Next article
feedback
Top