The Japanese Journal of Urology
Online ISSN : 1884-7110
Print ISSN : 0021-5287
ISSN-L : 0021-5287
A STUDY ON PREDICTING FACTORS OF THE EFFECT OF INTRA-ARTERIAL INFUSION CHEMOTHERAPY IN PATIENTS WITH BLADDER CANCERS
The Usefulness of In-Vitro Chemisensitivity Test Measuring Intracellular ATP Contents (ATP Assay)
Shin-ichiro YamadaYoshinori FujimotoKazutoshi IsogaiTakashi DeguchiShinichi NezasaShogo KawamotoYasuyuki NishidaMasayoshi TamakiHidetoshi EharaYoshito TakahashiManabu OkanoToshimi TakeuchiYukimichi KawadaTakuji Tanaka
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1994 Volume 85 Issue 10 Pages 1528-1533

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Abstract

We studied a relationship between in vitro sensitivity of the tumors to anti-cancerous drugs and histopathological effectiveness of an intra-arterial infusion chemotherapy in 15 patients with bladder cancers. The in vitro sensitivity test was performed by measuring intra-cellular ATP contents (ATP assay). The intra-arterial chemotherapy were performed by injecting methotrexate (MTX), adriamycin (ADM) and eisplatin (CDDP) from the internal iliac artery. When the intra-cellular ATP contents of the tumor cells treated with an anti-cancerous drug decreased to less than 50% of the untreated tumor cells, the tumor was evaluated as sensitive to the drug. The effectiveness of the chemotherapy were histipathologically evaluated by a pathologist according to the response criteria for bladder cancer treatment. When the histopathological responses of higher than grade 2 were observed in the tumor, the chemotherapy was evaluated as effective. In 8 of 9 tumors sensitive to ADM, chemotherapy were effective histopathologically and in all 6 tumors resistant to ADM, histopathological response of the chemotherapies were poor. The overall coincidence ratio between sensitivity to ADM and the histopathological effectiveness of the chemotherapy was 93%, showing statistically significant correlation. In 7 of 12 tumors sensitive to CDDP, the chemotherapies were effective and in 2 of 3 tumors resistant to CDDP, the chemotherapies were ineffective. Although the overall coincidence ratio between the sensitivities to CDDP and chemotherapeutic effectiveness was 60%, there was no significant correlation between them. In 7 of 8 tumors sensitive to both of ADM and CDDP, the chemotherapies were effective and in 6 of 7 tumors resistant to at least one of them, the chemotherapies were ineffective. The chemotherapies were significantly more effective on the tumors sensitie to ADM and CDDP than on those resistant to at least one of them. Therefore, the ATP assay to assess the in vitro chemosensitivity of the tumor would be a potential tool to predict the chemotherapeutic effectiveness in bladder cancers.

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