1995 Volume 5 Issue 4 Pages 141-148
As recently reported, a strong stimulation of noninfected CD4+-positive H9- cells by Sarcocystis gigantea (Syn. S. ovifelis) extract (SGE) was observed using the lymphocyte proliferation assay. After SGE-prestimulation, HIV-infected H9+ cells showed an exacerbation of the virus replication. In the present study, we investigated the reactivity of HⅣ-infected human monocytes by SGE. The highly sensitive p24 core profile ELISA was used to examine directly the amount of HⅣ produced. Experiments were performed using the permanent monocytic cells U937. Permanent incubation as well as preincubation with SGE before virus infection were able to stimulate HIV expression in all the cells. In U937 cells, the virus release per cell was 64 times higher in permanent stimulation with 320μg SGE compared to controls and 9 times higher in case of prestimulation.