Effects of a Sarcocystis gigantea Extract (SGE) on the Replication of Human Immunodeficiency Virus (HIV)

U. DRÖSIGK; H. -J. TIETZ; F. PÖZSCH; D. SCHOLZ; T. H. HIEPE

doi:10.32268/jprotozoolres.5.4_141

Effects of a Sarcocystis gigantea Extract (SGE) on the Replication of Human Immunodeficiency Virus (HIV)

U. DRÖSIGK, H. -J. TIETZ, F. PÖZSCH, D. SCHOLZ, T. H. HIEPE

著者情報

U. DRÖSIGK
Institute of Parasitology and Tropical Veterinary Medicine, Free University of Berlin, Königsweg 67, D-14163 Berlin, Germany
Department of Dermatolog and Institute of Virology, Humboldt University of Berlin (Charité), Schumannstraße 20/21, D-10117 Berlin, Germany
H. -J. TIETZ
Institute of Parasitology and Tropical Veterinary Medicine, Free University of Berlin, Königsweg 67, D-14163 Berlin, Germany
Department of Dermatolog and Institute of Virology, Humboldt University of Berlin (Charité), Schumannstraße 20/21, D-10117 Berlin, Germany
F. PÖZSCH
Institute of Parasitology and Tropical Veterinary Medicine, Free University of Berlin, Königsweg 67, D-14163 Berlin, Germany
Department of Dermatolog and Institute of Virology, Humboldt University of Berlin (Charité), Schumannstraße 20/21, D-10117 Berlin, Germany
D. SCHOLZ
Institute of Parasitology and Tropical Veterinary Medicine, Free University of Berlin, Königsweg 67, D-14163 Berlin, Germany
Department of Dermatolog and Institute of Virology, Humboldt University of Berlin (Charité), Schumannstraße 20/21, D-10117 Berlin, Germany
T. H. HIEPE
Institute of Parasitology and Tropical Veterinary Medicine, Free University of Berlin, Königsweg 67, D-14163 Berlin, Germany
Department of Dermatolog and Institute of Virology, Humboldt University of Berlin (Charité), Schumannstraße 20/21, D-10117 Berlin, Germany

ジャーナルフリー

1995 年 5 巻 4 号 p. 141-148

DOI https://doi.org/10.32268/jprotozoolres.5.4_141

詳細

抄録

As recently reported, a strong stimulation of noninfected CD4⁺-positive H9^- cells by Sarcocystis gigantea (Syn. S. ovifelis) extract (SGE) was observed using the lymphocyte proliferation assay. After SGE-prestimulation, HIV-infected H9⁺ cells showed an exacerbation of the virus replication. In the present study, we investigated the reactivity of HⅣ-infected human monocytes by SGE. The highly sensitive p24 core profile ELISA was used to examine directly the amount of HⅣ produced. Experiments were performed using the permanent monocytic cells U937. Permanent incubation as well as preincubation with SGE before virus infection were able to stimulate HIV expression in all the cells. In U937 cells, the virus release per cell was 64 times higher in permanent stimulation with 320μg SGE compared to controls and 9 times higher in case of prestimulation.

責任著者(Corresponding author)

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