Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii

抄録

To generate transgenic mice carrying a protozoan gene, 3.1 kb DNA fragments carrying the CAG promoter ligated with a protozoan gene encoding a major surface antigen SAG-1 (p30) of Toxoplasma gondii, were microinjected into one of pronuclei of embryos of C57BL/6J and BALB/c mice. The embryos were transferred to the oviducts of ICR pseudopregnant recipients. The transgene was detected by polymerase chain reaction in DNA sequence purified from tissue biopsies. Out of 159 mice that developed from injected eggs two p30-founders (BALB/c and C57BL/6J) were obtained. The transgene was not detected in 52 pups of F1 progeny of C57BL/6J founder. However, 3(17.6%) out of 17 F1 progenies from BALB/c founder were found to have p30 gene and they inherited the transgene to 39(55.7%) of 70 F2 progenies. These results may afford the opportunity to study the role of SAG-1 (P30) gene in Toxoplasma gondii infection.