抄録
Several recent studies have suggested that radiation-induced genomic instability involved in radiation carcinogenesis. However the mechanisms of radiation-induced genomic instability have not been clarified. The generation and repair of radiation-induced initial DNA damages in cells have been recently examined by enumeration of phosphorylated histone H2AX (γ-H2AX) foci. This result showed that initial DNA damage induced by a certain amount of dose might not be able to be restored and, there is a possibility remaining for a long time. Although the biological meaning of remains of this initial DNA damage has not been clarified yet, we thought that remaining of initial DNA damage may relate to the induction of the radiation carcinogenesis. In this study, we investigated that time course analysis of radiation -induced initial DNA damage based on γ-H2AX and ATM focus formation in primary human fibroblasts.
