Experiments were done on 10 mongrel dogs to observe the histological changes of liver, spleen, lung, and kidney after 3-hour cardipulmonary bypass (CPB) by apulsatile pump with fluorocarbon emulsion.
Dogs were anesthetized and thorachotomy was done bilaterally, after connected to the circuit, the 3-hour CPB was done.
As a priming fluid, fluorocarbon emulsion (Fluosol DA 35% The green cross corporation, Japan) was used instead of homologous blood.
After 3-hour CPB, dogs were sacrificed and their tissues were fixed with 10% formalin and stained bi Hematoxylin-Eosin stain or PAS stain.
Histological changes in the liver were most remarkable. Vacuoles wich were not stained by neither stain were seen in hepatocytes or Kupffer's cells. Sinusoid was slightly enlarged but no necritic changes were seen.
Monocytes in spleen contained fine granules or small vacuoles.
In the lung, alveolar walls were slightly edematous and kidney maintained it's microstructure.
These findings were similar to the findings after 2 days of exchange transfusionwith fluorocarbon to rats and monkeys.
