Characterization of different DLC and DLN electrodes for biosensor design

R. MAALOUF; Nicole JAFFREZIC-RENAULT; O. VITTORI; M. SIGAUD; Y. SAIKALI; H. CHEBIB; A. S. LOIR; F. GARRELIE; C. DONNET; T. TAKENO; T. TAKAGI

doi:10.2978/jsas.18.31

Paper

Characterization of different DLC and DLN electrodes for biosensor design

R. MAALOUF ^†, Nicole JAFFREZIC-RENAULT ^†, O. VITTORI ^†, M. SIGAUD ^†, Y. SAIKALI ^†, H. CHEBIB ^†, A. S. LOIR ^†, F. GARRELIE ^†, C. DONNET ^†, T. TAKENO ^†, T. TAKAGI ^†

Author information

R. MAALOUF ^†
Center of Electrical Engineering of Lyon, CEGELY, Ecole Centrale Lyon
Nicole JAFFREZIC-RENAULT ^†
Center of Electrical Engineering of Lyon, CEGELY, Ecole Centrale Lyon
O. VITTORI ^†
Laboratory of Analytical Electrochemistry, Claude Bernard University
M. SIGAUD ^†
Laboratory of Analytical Electrochemistry, Claude Bernard University
Y. SAIKALI ^†
Laboratory of Chemistry, Lebanese University
H. CHEBIB ^†
Laboratory of Chemistry, Lebanese University
A. S. LOIR ^†
Laboratory of Signal Processing and Instrumentation
F. GARRELIE ^†
Laboratory of Signal Processing and Instrumentation
C. DONNET ^†
Laboratory of Signal Processing and Instrumentation
T. TAKENO ^†
Institute of Fluid Science, Tohoku Universiy
T. TAKAGI ^†
Institute of Fluid Science, Tohoku Universiy
Author contributions

†These authors contributed equally: R. MAALOUF, Nicole JAFFREZIC-RENAULT, O. VITTORI, M. SIGAUD, Y. SAIKALI, H. CHEBIB, A. S. LOIR, F. GARRELIE, C. DONNET, T. TAKENO, T. TAKAGI

Keywords: Diamond-Like Carbon, Glucose oxidase, Amperometric measurements, Biosensor

JOURNAL FREE ACCESS

2006 Volume 18 Issue 1+2 Pages 31-36

DOI https://doi.org/10.2978/jsas.18.31

Details

Abstract

Diamond-Like Carbon and Carbon-Like Nanocomposite electrodes, novel materials in the field of biosensors, made with different ratio of sp³/sp² carbon hybridization or doped with elements such as Ni, Si and W, were characterized electrochemically by cyclic voltammetry and by amperometric measurements towards hydrogen peroxide. SiCAr1 and SiCNi5% were chosen as sensitive transducers for elaboration of amperometric glucose biosensors. Immobilization of glucose oxidase was carried out by cross-linking with glutareldehyde. Measurements were made at a fixed potential + 1.0 V in 40 mM phosphate buffer pH 7.4. SiCAr1 seems to be more sensitive for glucose (0.6875 μA/mM) then SiCNi5% (0.3654 μA/mM). Detections limits were respectively 20 μM and 30 μM. Michaelis-Menten constants for the two electrodes were found around 3 mM. 48% and 79% of the original response for 0.5 mM glucose remained respectively for both electrodes after 10 days.

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