In order to analyze physiological aspect of motion sickness, an experiment was carried out with artificially generated motion sickness using revolving chair. As the physiological measures, power spectra of ECG R-R interval series, EGG, and R-wave Plethysmogram onset asynchrony (RPA) were used. Effects of administrated stomach medicine were also studied. In the motion sickness condition, changes of autonomic indices were observed. Importance of autonomic aspect in assessment of motion sickness was pointed out.