マウスのパラチフスB菌感染に関する実験的研究

第6報 マウスに種々の前処置を行つた場合について

抄録

From the first to fifth reports successively, the establishment of oral infection of paratyphoid B was confirmed experimentally using various methods. The following observations were obtained. The sign of sickness appeared in almost all mice. The discovering of bacilli harboring in mice organs continued until the 15th week. The blood picture changed. The serum agglutinin titer against paratyphoid B O and H antigen raised significantly. All strains of mice examined were infected though grade varied with strain. Histological examination showed several proofs of infection also.
In this report, mice were treated with some methods before infection to change the mode of infection, and the following results were obtained.
1) When carbon tetrachloride was injected intraperitoneally before infection, more infecting bacilli were discovered in mice organs.
2) When tripanblue was combined with carbon tetrachloride pretreatment, many mice died of paratyphoid B bactremia.
3) After Bordetella pertussis intracerebral inoculation, paratyphoid B oral infection was established in almost all mice.
4) When Freund's adjuvant prepared by mice intestines cut out from duodenum to rectum and that contents had been washed out was injected intramuscularly for sensitization to infection site namely intestines, the rate of bacilli retention in organs rather reduced.
5) The quantity of dead tubercle bacilli contained in Freund's adjuvant, the frequency of adjuvant injection to mice as pretreatment, and the interval between abjuvant injection and paratyphoid B infection were changed respectively.
The change of quantity of dead tubercle bacilli showed no influence upon experiment. The prolongation of interval between adjuvant injection and bacilli administration resulted in increase of bacilli harboring in organ culture.