腸内細菌の薬剤耐性

ε₁₅およびε₃₄ phageによるR因子の導入について

抄録

Transmissible drug-resistance (R) factor was transduced in the system of SalmonellaE group with phage epsilon, and its transduction rate was 10^-8.
The transduction rate of R factor with epsilon phage that had existed as prophage in R⁺host cell and was induced by UV irradiation increased about 10² times than that of ordinary transduction.
The R factor carrying TC resistance marker was consistently segregated by transduction with epsilon phage.
The R⁺transductant was not lysogeniged by transducing epsilon phage, indicating non-lysogenic conversion and non-immunity toward the transducing epsilon phage.
The R factor of transductant was non-transmissible by conjugation and not eliminated by acriflavine treatment. But the R factor of transductant was transduced again by epsilon phage that had existed as prophage in R⁺transductant or that was newly infected to R⁺transductant.
The R factor of R⁺transductant of S. chittagongwas transmissible by conjugation at 10⁶times lower frequency than that of R factor that was transferred by conjugation, and was eliminated by acriflavine treatment in low frequency.
In transduction of R factor R⁺ transductant with the same epsilon phage that had transduced high frequency transductant (HFT) was obtained and its transduction rate was 10^-2.
The transmission of R factor to R⁺transductant was higher than that to R^-or R⁺cell to which R factor was transmitted by conjugation.