抗炭疽沈降素血清に対するブドウ球菌の反応性
II. 炭疽菌とブ球菌の共通抗原について
1962 年 17 巻 5 号 p. 330-333
詳細
1962 年 17 巻 5 号 p. 330-333
In a previous paper of this series we reported that Staphylococcus aureus was reacted to anti-anthrax precipitin serum.
This report describes a method of fractionation of crude common antigenic substance between Bacillus anthracis and Staph. aureus 209 p, and the results of the absorption test for the relation between antigenic structure of these crude common antigen.
Each fraction was obtained as follows; The heat dried cells were suspended in physiologic saline solution and extracted at 37°C for 3 hours.
HC1-fraction was precipitated from the extract by the addition to pH 3.8 with N-HCl. After removal of the HC1-fraction, the CuSO4 fraction was precipitated from the supernatant by the addition of CuSO4 to make the 10 per cent. After removal of the CuSO4 fraction, the supernatant was treated with Sevag method and Sevag-fraction was precipitated from the gel part by the addition of 4-fold of methanol and reprecipitated with trichloroacetic acid.
The resultant supernatant was dialysed with distilled water and lyophilysed. It was polysaccharide fraction.
The results of the absorption test in these fraction, the common antigenic substance was present in HCl-fraction and Sevag fraction.
