実験チフス症における内毒素の意義について

I. 腸炎菌各種変異株からの内毒素の抽出ならびに内毒素のそれら菌株のマウス感染に及ぼす影響

抄録

To analyse the role of endotoxin in the pathogenesis of experimental typhoid in mice, (1) lethal toxicities to mice of heat-killed bacteria, (2) the amount of lipopolysaccharide (endotoxin), and (3) the influence of endotoxin on infection of mice were investigated usingSalmonella enteritidisstrains with different virulence.
Heat-killed cells of a rough mutant 11Rx were somewhat less toxic than those of its wild type strain No.11 (high-virulent) and an S-type strain ‘Jena’(almost non-virulent). A galactose-sentive (epimeraseless) mutant of No.11, 11-1-M, and its galactose-resistant mutant, 11-1-TW, were found to have extremely low toxicities and almost no virulence for mice.
The yield of lipopolysaccharide obtained by Westphal's procedure following ultracentrifugation was poor in the strains of low toxicity, but no remarkable difference in the lethal effect for mice was found among endotoxins extracted from various strains.
Influence of the endotoxin on infection with those strains was studied by observation of mortality and quantitative cultivation of the blood, peritoneal fluid, spleen and liver at various intervals after challenge and the simultaneous administration of endotoxin. Thein vivomultiplication of strains ‘Jena’ and 11-1-M was not enhanced by endotoxin, but strain 11-1-TW could markedly multiply in the peritoneal fluid and spleen when inoculated with endotoxin. Furthermore, strain 11 Rx, which was low-virulent compared with strain No.11, showed as high as the virulence that of strain No.11 when given with endotoxin.