感作処置による実験的肺アスペルギルス症について
1963 年 18 巻 4 号 p. 178-183
詳細
1963 年 18 巻 4 号 p. 178-183
In view of the difficulty to establish a localized pulmonary Aspergillus infection in experimental animals, the authors attempted to produce the infection in rabbits with the aid of sensitization by the cell components ofAspergillus fumigatus.
Sensitization of rabbits was done very effectively by the repeated intramuscular injection of the whole cell homogenate, cell wall preparation and lipopolysaccharide fraction obtained from a certain strain ofA. fumigatus. Infection of the animals was followed by the intrapulmonal injection of spores of the fungus. This treatment for sensitization proved to be a very important prerequisite for producing lesions in the lung, being much severer in the sensitized animals than in the non-sensitized or non-treated.
The physical condition of emulsion of the sensitizing materials was known to be also important in sensitizing animals; a water in oil (w/o) emulsion was much more effective than a oil in water (o/w) emulsion, resulting in the development of severer lesions. However, the physical condition of spore suspension seemed not significant in this point; in the rabbits sensitized with a w/o emulsion of the sensitizing materials, lesions were produced by both w/o and o/w emulsions of spores, whereas when sensitized in o/w emulsion, developed lesions only by a o/w spore emulsion.
Concerning the activities of induction of lesions, the cell wall preperation was almost same as the whole cell homogenate, whereas the lipopolysaccharide fraction was much weaker than the above two, even than the non-treated controls.
Sensitization was also tried to establish by means of endotracheal inoculation of spores in physiological saline solution and infection was done by endotracheal route, too. These treatments, however, produced more extensive and slighter lesions.
Thus, it may be concluded that the most effective method for establishing a localized pulmonary aspergillosis in rabbits consists of a combination of sensitization of the animals by repeated intramuscular injection of the whole cell homogenate or cell wall preparation ofA. fumigatusin a w/o emulsion and the subsequent intrapulmonal inoculation of spores in a w/o emulsion.
