Studies on the Immunospecific and Non-specific Substance of B. anthracis

II. Preparation of Immunospecific Substance

Abstract

1) From the heated cell extract of B. anthracis, an antigenic substance was isolated, through trichloracetic acid treatment, the removal of lipids by ether, aceton fractionation and zone electrophoresis, which was suggested to be homogeneous by the analytical centrifugation and agar diffusion test.
2) This substance reacts with anti-anthracis serum in 32000-fold dilution, but not with anti cereus and anti-subtilis serum; it is specific in the above sense.
3) This substance was positive in Biuret reaction and Folin reaction and showed the absorbtion of maximum in 280 mu, while negative in sugar and nucleic acid reaction.
These facts strongly suggest this substance to be protein like.