We examined for the productive conditions of the extracellular antigenic glycopeptide which was excreted by Aspergillus fumigatus in culture medium and obtained the following results.
Powerful antigenicity of culture filtrate of Asp. fumigatus growing on the sabouraud medium, yeast extract medium and the other synthetic media was observed after 10 days incubation especially, and it showed with appearance of a glycopeptide on the paperelectrophoretic pattern of 80% MeOH fraction of the filtrate after 3 or 4 days incubation.
Total carbohydrate of the 80% MeOH fractions increased with development of culture and their sugar components were composed of galactose and mannose.
From the results of the precipitin activity of the fraction eluted with water on Sephadex G-50 column, it was found that polysaccharide fraction showed high activity but protein fraction was very weak.