Breeding Science
Online ISSN : 1347-3735
Print ISSN : 1344-7610
Research papers
Development of Microsatellite Markers in Melon (Cucumis melo L.) and Their Application to Major Cucurbit Crops
Naoki ChibaKeita SuwabeTsukasa NunomeMasashi Hirai
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2003 Volume 53 Issue 1 Pages 21-27

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Abstract

We developed 31 sets of melon (Cucumis melo L.) microsatellite markers in this study. Primers for these markers were designed from clones containing simple sequence repeats (SSRs) of (GA/CT)n or (GT/CA)n isolated from a melon genomic library. These microsatellite loci were examined for the detection of alleles among twelve lines and cultivars from six varieties of C. melo. A total of 28 marker loci showed polymorphism by 2.0 % agarose TBE gel separation. And these markers were also examined for cross-species amplification applicable to nine other species in Cucurbitaceae. Among them, 13 marker loci were commonly detected within the major cucurbit crops, cucumber, pumpkin and watermelon using the present primer sets. The species to which the melon microsatellite markers would be most applicable was Momordica charantia (24 markers), followed by Cucumis sativus (20 markers) and Cucurbita maxima (18 markers). These conserved microsatellite loci could be used as anchor markers in studies on synteny in Cucurbitaceae.

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© 2003 by JAPANESE SOCIETY OF BREEDING
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