We developed a rice (Oryza sativa) transformation system using silicon carbide whiskers (SCW). The scutellar tissues of rice embryos obtained from mature seeds were vortexed in liquid medium containing plasmid DNA and SCW. The embryos treated with a plasmid DNA, pAct1-F containing the β-glucuronidase (gusA) gene, transiently expressed GUS activity at a mean number of 302 spots per 250 mg sample (around 20 embryos) in optimal conditions for DNA delivery. On the other hand, the bialaphos-resistant calli were obtained from embryos treated with two plasmid DNAs, pAct1-F and pDM302 containing the bar gene, of which product confers resistance to bialaphos. Some of the resistant calli also expressed GUS activity. Fertile transgenic rice plants were regenerated from them. The integration and inheritance of bar gene were confirmed by Southern analysis of R0 and R1 plants.
Japanese Society of Breeding