ポリマー薄膜を用いた三次元マイクロ流路デバイスの研究

再生医療のための人工毛細血管の開発

抄録

This paper presents a three-dimensional microfluidic network made of thin mesoporous biodegradable polymer membrane, which realizes rapid transfer of liquid and gas between inside and outside of the microchannel. This is a first prototype of the “artificial blood capillary device” we have proposed to regenerate large tissues and organs in vitro. Here, we prepared porous poly-lactic acid membrane using spincoating following phase separation, and examined the relation of solution composition to pore size and density. We checked the permeability of the prepared membrane for three different dyes which have varying molecular weight. We also investigated the biocompatibility of the membrane by culturing human endothelial cells on the membrane. At last, we successfully fabricated a microfluidic network using membrane micro embossing (MeME) process, and confirmed the size-selective transfer of micro-beads through the microchannel walls.