臨床化学
Online ISSN : 2187-4077
Print ISSN : 0370-5633
ISSN-L : 0370-5633
脂質専用標準血清の作製に関する検討
栢森 裕三手登根 稔片山 善章
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1990 年 19 巻 2 号 p. 176-182

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We herein describe a procedure for manufacturing the human serum based control specific. f or lipid assay, applying the precipitation method by Burstein et al.
The control serum is prepared by reconstituting a lyophilized LDL and VLDL depleted serum with distilled water and adding a liquefied LDL and VLDL components to it when in use.
The advantageous point this procedure is that the concentration of each lipid component can be changed according to the requirement. The control serum can equally be compared with fresh human serum in its transparency, electrophoretic characteristics and recovery of lipids by ultracentrifugal analysis.
Furthermore the enzymatic analyses of triglycerides, total cholesterol and phospholipids in the control serum the similar reaction kinetics as those of fresh human serum.
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