Abstract
Cell walls play an indispensable role in the growth and development of plants. Since the mechanical properties of cell walls limit the flexibility of cells to change their size and shape, the measurement of mechanical properties is an important technique to monitor the physiological state of plant tissues. Stress-relaxation method and creep extension method have been widely used with technical progress of mechatronics. Basic methods, theory and characteristics of two methods were summarized by using the mechanical model and equation. Technical notes for the measurement of creep extension analysis were also presented based on the experiences in the study of root cell walls. Special note on the technique of clamping a small root tip at 1-mm distance was described with figures. Several technical problems were pointed out for further improvement of technique hoping the detection of molecular movement in the expanding cell walls.
