植物の生長調節
Online ISSN : 2189-6305
Print ISSN : 1346-5406
研究ノート
細胞内の温度を可視化する
─細胞内温度イメージングの実践─
稲田 のりこ岡田 松美林 晃之内山 聖一
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ジャーナル フリー

2021 年 56 巻 2 号 p. 112-116

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Temperature is the most important physical parameter that fundamentally affects cellular metabolism and functions. Analysis of intracellular temperature is expected to provide new information of cellular condition and metabolism. We have developed a method to analyze intracellular temperature changes using fluorescent polymeric thermometer (FPT) and fluorescence lifetime imaging microscopy (FLIM). Using this method, we found that there is a temperature difference >1°C within a mammalian cultured cell, and the temperature in the nucleus and in the area near mitochondria and centrosome is higher than the other area in the cytoplasm. While we originally adapted FLIM for accurate intracellular temperature measurement, fluorescence microscope or confocal laser scanning microscope can also be used. In this technical note, we describe the brief protocol for intracellular imaging of mammalian cultured cells using FPT with fluorescence microscope or confocal laser scanning microscope. We also discuss the future research directions using intracellular temperature imaging.

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