Structural determination of novel strigolactones (SLs) has been contributed to the elucidation of biosynthetic and metabolic pathways of SLs. Since SLs are only minor components of plant metabolites released to the rhizosphere, SLs must be separated from the other metabolites in root exudate with high purities and high recovery rates. Here, I would like to introduce some tips on how to improve the recovery rate of SLs from the root exudate of hydroponically grown plants.