11 イネDWARF遺伝子のクローニングとその発現に対する光の影響
2001 年 36 巻 p. 33-34
詳細
2001 年 36 巻 p. 33-34
We found that light seems to enhance the conversion of 6-deoxocastasterone to castasterone in rice seedlings. So we cloned the rice DWARF gene which is involved in the conversion from 6-deoxocastasterone to castasterone, and compared the effect of light quality on its expression by the semi-quantitative RT-PCR. Total RNAs were extracted from whole shoots and whole roots of rice grown in dark for 1 week, and then under different light conditions (white, blue, red, far red, and dark) for 24 hours. However, different light conditions caused no significant alteration in the expression of the rice DWARF gene. We are now attempting to analyze younger, rapid-growing regions of the rice seedlings.
