Sporicidal Effects of Excess Liquid from Sporicidal Wipes against Clostridioides Difficile Spores

Abstract

The sporicidal effect of excess liquid from sporicidal wipes was evaluated in comparison to sodium hypochlorite against the spores of three strains of Clostridioides difficile under dirty (3.0 g/L albumin plus 3.0mL/L sheep erythrocytes) conditions. The wipes were soaked in 20 mL distilled water for 4 min. The excess liquid from the soaked wipes was subsequently used in the experiment. Sodium hypochlorite of 0.1% (1,000 ppm) did not exhibit adequate sporicidal activity even after 10 min of contact, whereas sporicidal wipes killed the spores within 5 min. Our results suggest that using sporicidal wipes is effective for disinfecting environments contaminated with C. difficile spores.