To measure ion transport through a membranous labyrinth, we devised a micro chamber unit consisting of a micro chamber, a circulation pump and a storage tank. A cellulose triacetate membrane was inserted to divide the chamber into two circuits. One circuit was filled with physiological saline, the other with 5% glucose solution. We measured the passive transport of Na+ through the membrane, then tested it under various conditions simulating those in the human membranous labyrinth. The suitable conditions were 7 mm for the diameter of the chamber and 10 Ml/min for the circulation rate.